EuroMRD dPCR NHL 2026

EuroMRD dPCR NHL EQA scheme 2026

Task 8: dPCR for MRD quantification in NHL

The use of dPCR for the quantification of minimal residual disease (MRD) in lymphoid malignancies has seen a marked increase. Within the EuroMRD Consortium, a dPCR quality assessment scheme was set up and utilized for the standardization and establishment of guidelines for dPCR based MRD quantification in lymphoma. dPCR is performed on samples based on the same DNA as provided for Task 4A/B of the EuroMRD NHL EQA scheme. dPCR analysis should be performed according to the instructions provided by the organizers.

Task 8A: IGH::BLC2 dPCR in Follicular Lymphoma

Task 8A consists of dPCR based MRD quantification of IGH::BLC2 major or minor (MBR or mcr) breakpoints on provided samples.

Task 8B: IGH::CCND1 and IGH dPCR in Mantle Cell Lymphoma

Task 8B consists of the analysis of two provided sequences, an IGH::CCND1 translocation and an IGH gene rearrangement. For both targets, a primers/probe combination must be designed and dPCR analysis performed on follow-up (FU) samples.

Task 8A is issued for the spring and Task 8B for the autumn EQA round.

Performance evaluation and report

Certificates of participation in EQA rounds will be distributed after the EuroMRD meeting of the same calendar year.

After release of the certificates, participants can submit an appeal if they feel that a mistake was made in the assessment of their performance. If applicable, certificates will be adapted based on received appeals.

EQA Educational meeting

During the ESLHO week in November, the annual EuroMRD meeting is organized. At this meeting, the results of all EuroMRD EQA schemes are shown (pseudonymized) and discussed with all EuroMRD participants, in small discussion groups as well as during plenary sessions.

Registration and participation fee

The EuroMRD dPCR NHL EQA scheme is only available for participants of the EuroMRD Consortium that are part of the NHL section. The participation fee is € 300, regardless of whether you participate in one or both rounds. In addition, the EuroMRD annual fee is charged once to every EuroMRD participant.

Organization

The dPCR NHL EQA scheme is organized by ESLHO (EQA Program Coordinator: Prof. Dr. Jacques J.M. van Dongen) in collaboration with the EuroMRD EQA Committee (dPCR NHL scheme lead: Dr. Daniela Drandi). The members of the EuroMRD EQA Committee are participants of the EuroMRD Consortium. The EuroMRD subject-matter experts provide support for:

• Pre-round: Case selection, determination of consensus results

• Post-round: Data analysis, performance evaluation, reporting

Details on the task division can be found below.

Task division