EuroFlow LST 2026
Lymphoid Screening Tube (LST) EQA scheme
Aim of the scheme
The LST scheme is intended for laboratories that want to assess their performance in utilizing EuroFlow’s hematologic-malignancies diagnostic and classification panels and relevant EuroFlow standard operating procedures (SOPs) in their routine diagnostics.
This scheme evaluates your ability to:
prepare samples
set up the flow cytometer
analyze the FCS files
You will stain 3 healthy donor peripheral blood samples sourced locally at your laboratory (note that no samples will be provided by ESLHO) with the LST antibody panel and analyze the data. You will report the median fluorescence intensity (MedFI) values via the ESLHO EQA Portal.
Your results will be compared to the reference values (Kalina et al., 2015, 2019) and to the results of the other participants in the round.
Two rounds of the LST scheme are offered in 2026: one in spring and one in autumn.
Performance and report
After each round, participating laboratories will be provided with an EQA certificate showing their performance as compared to the reference values and to other laboratories. In addition to the performance report, specific comments or recommendations on how routine practice could be improved may be provided.
Further details on data analysis, how participants’ performance is evaluated, and on the contents of the report can be found in the document “Scheme information” available on the Resources page.
Educational meeting
All EuroFlow schemes’ EQA rounds offered in 2026 will be concluded with an online educational meeting, which will include all rounds performed throughout the year. During the meeting, the rounds’ results will be shown (anonymized), possible problems and pitfalls will be discussed, and there will be the opportunity to receive direct feedback from the experts involved. More information regarding the educational meeting, including dates and times, will be announced at the end of 2026.
Organization
The LST scheme is organized by ESLHO in collaboration with the EuroFlow EQA Committee. The EuroFlow EQA Committee is composed of members of the EuroFlow Consortium (www.euroflow.org). The laboratory at the Charles University, Prague, Czech Republic, operates as the leading expert laboratory of the LST scheme, with Prof. Dr. Tomáš Kalina in the role of lead expert.
Further details on the task division of involved experts can be found in the document “Scheme information” available on the Resources page.
Participation fees
Participation in one round: € 270,-
Participation in both rounds: € 490,-
Participation with an additional certificate (e.g. for additional instruments): € 110,- per additional certificate.
Participation is free for participants of the EuroFlow Consortium.