EuroFlow LST 2025
Lymphoid Screening Tube (LST) EQA scheme
Aim of the scheme
The LST scheme is intended for laboratories that want to assess their performance in utilizing EuroFlow’s hematologic-malignancies diagnostic and classification panels and relevant EuroFlow standard operating procedures (SOPs) in their routine diagnostics.
This scheme evaluates your ability to:
prepare samples
set up the flow cytometer
analyze the FCS files
You will stain 3 healthy donor peripheral blood samples sourced locally at your laboratory with the LST antibody panel and analyze the data. You will report the median fluorescence intensity (MedFI) values via the ESLHO EQA portal.
Your results will be compared to the reference results (Kalina et al., 2015, 2019) and to the results of the other participants in the round.
Two unique rounds of the LST scheme are offered in 2025: one in spring and one in autumn.
Performance and report
After each round, participating laboratories will be provided with an EQA certificate showing their performance as compared to the reference values and to other laboratories. In addition to the performance report, specific comments or recommendations on how routine practice could be improved may be provided.
Further details on data analysis, how participants’ performance is evaluated, and on the contents of the report can be found in the document “Scheme information” available on the Resources page.
Educational meeting
All EuroFlow schemes’ EQA rounds offered in 2025 will be concluded with an educational meeting, which will include all rounds performed throughout the year. Participation is free of charge for two representatives per participating laboratory. During the meeting, the rounds’ results will be shown (anonymized), possible problems and pitfalls will be discussed, and there will be the opportunity to receive direct feedback. More information regarding the educational meeting will be announced on the ESLHO EQA Portal.
Organization
The LST scheme is organized by ESLHO in collaboration with the EuroFlow EQA Committee. The EuroFlow EQA Committee is composed of members of the EuroFlow Consortium (www.euroflow.org). The laboratory at the Charles University, Prague, Czech Republic, operates as the leading expert laboratory of the LST scheme, with Prof. Dr. Tomáš Kalina in the role of lead subject-matter expert.
Further details on the task division of subject-matter experts can be found in the document “Scheme information” available on the Resources page.
Participation
ESLHO offers the LST 2025 rounds free of charge to participants of the EuroFlow Consortium.